| Autor: |
Iqbal, Aneela, Khan, Raham Sher, Khan, Mubarak Ali, Gul, Karim, Aizaz, Muhammad, Usman, Muhammad, Arif, Muhammad |
| Zdroj: |
Sugar Tech; Feb2023, Vol. 25 Issue 1, p168-176, 9p |
| Abstrakt: |
In vitro micropropagation using transverse thin cell layer (tTCL), very small and thin section of explant, has now emerged as a useful tool in plant tissue differentiation and regeneration. Sugarcane, a difficult-to-regenerate crop, lacks an efficient regeneration system. The present study evaluated the TCL technique as a procedure for somatic embryo production and plantlet regeneration of sugarcane plant. Explants from young leaf whorls (tTCLs) were cultivated in MS culture medium supplemented with 3% sucrose and different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and incubated under dark condition at 25 + 2 °C for callus formation. The innermost layer of the explants was found more responsive to callogenesis than the outer layers of the tTCLs. The explants cultured on 2.0 mg/l 2,4-D produced good-quality callus as compared to other concentrations. The calli were further evaluated for regeneration. MS medium supplemented with 2.0 mg/l BAP (Benzyl aminopurine) and 0.2 mg/l NAA (naphthalene acetic acid) was found suitable where 100% calli regenerated maximum number of shoots per callus mass (25 ± 1.6) with highest shoot length of 6 ± 0.5 cm. The highest number of root emergence (28.2 ± 0.8) and maximum root length (2.8 ± 0.09 cm) were achieved on MS medium supplemented with 4.0 mg/l NAA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink