| Autor: |
Yashwant, Rasalkar Sandhya, Thomas, Dyna Susan, Manoharan, Chitra, Nongthomba, Upendra, Sivaprasad, Vankadara, Gopalapillai, Ravikumar |
| Zdroj: |
Applied Entomology & Zoology; Feb2023, Vol. 58 Issue 1, p85-92, 8p |
| Abstrakt: |
Silkworms and other insects confer innate immunity by expressing antimicrobial peptides (AMPs) through the induction of Toll and IMD pathways. Cecropin B, an AMP from Bombyx mori L. (Lepidoptera: Bombycidae), has a broad range of antibacterial activity against both Gram-positive and -negative bacteria. Bacterial flacherie is one of the severe diseases in silkworms. In an attempt to develop silkworm strains expressing antibacterial properties, a transgenic vector, piggyBac overexpressing the cecropin B gene, was constructed under its promoter. The vector had GFP under the control of the elongation factor (ELFα) promoter as a marker for screening transgenic silkworms. Transgenic silkworms were generated by microinjecting the piggyBac vector along with the helper vector into the silkworm eggs. The mRNA level of cecropin B in the fat body of transgenic lines was higher than the non-transgenic lines in response to Escherichia coli and Staphylococcus aureus infections. The overexpression of cecropin B was confirmed by Western blot analysis. Transgenic lines' mortality was significantly decreased compared to non-transgenic lines' post-bacterial infections. These results imply that overexpressing an endogenous AMP gene can enhance the resistance of silkworms in response to bacterial infections. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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