| Autor: |
PRIYANKA, B. V., NAGESHA, S. N., NAGARAJ, M. S., GIRISH, H. C., NAGESHA, N., GOVINDA, GEETHA, BHAVANI, P., KUMARASWAMY, R. V. |
| Předmět: |
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| Zdroj: |
Mysore Journal of Agricultural Sciences; Oct-Dec2022, Vol. 56 Issue 4, p349-357, 9p |
| Abstrakt: |
Research for the development of biological control agents as an alternative to agrochemicals for controlling plant pathogens has gained considerable attention for the acquirement of agricultural sustainability. Fusarium is a complex genus of ascomycete fungi that consists of plant pathogens of agricultural relevance. The genus Pythium is one of the most important groups of soil-borne plant pathogens, present in almost every agricultural soil and attacking the roots of thousands of hosts, reducing crop yield and quality. In the present study, an analysis of the bio-control attributes of Bacillus subtilis has been conducted and demonstrated the most effective antagonism against Fusarium spp. and Pythium spp. under in vitro conditions. Bacteria, especially members belonging to the genus Bacillus are well recognized for defending the plants against various phytopathogens due to their ability to produce a variety of secondary metabolites and siderophores. Iturin A gene was PCR amplified and showed 675bp length. The lipopeptide was extracted and quantified (O.D.280) from two strains T3 (35µg/ml) and T6 (18µg/ml). The extracted lipopeptide and cell-free extract (supernatant) of T3 and T6 strains of Bacillus subtilis were used at a concentration of 25µg/ml and 10µl/ml of media was used against Fusarium oxysporum and Pythium aphanidermatum, respectively to check the efficacy of antifungal activity. The antifungal activity of the bacterial isolates against pathogens was recorded and a growth inhibition zone was observed. It was observed that 4 cm radius in control without inhibition. The poison food technique method with lipopeptide of T3 and T6 strains showed 36.79 per cent and 51.46 per cent inhibition activity and cell-free extract of T3 and T6 strain showed 33.54 per cent and 52.63 per cent inhibition activity, respectively against Fusarium oxysporum. Also, lipopeptide of T3 and T6 strains showed 5.33 per cent and 11.46 per cent and cell-free extract showed 1.46 per cent and 1.25 per cent inhibition activity respectively against Pythium spp. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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