| Autor: |
MUTTAPPAGOL, MANTESH, VINAYKUMAR, H. D., HIREMATH, SHRIDHAR, NANDAN, M., BASHA, C. R. JAHIR, SHANKARAPPA, K. S., VENKATARAVANAPPA, V., REDDY, C. N. LAKSHMINARAYANA |
| Předmět: |
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| Zdroj: |
Mysore Journal of Agricultural Sciences; Oct-Dec2022, Vol. 56 Issue 4, p267-276, 10p |
| Abstrakt: |
Niger [Guizotia abyssinica Cass.] is one of the important minor oilseed crops grown in India. Its production is challenged by numerous diseases affecting production and quality, of which phyllody is emerging as constraint. During June 2021, niger plants exhibiting typical symptoms of phytoplasma infection were observed at the experimental field of Zonal Agricultural Research Station, Bengaluru (13.0801N,77.570E), Karnataka, India. The infected plants showed excessive proliferation of shoots, reduced leaf size and stunted growth leading, phyllody symptoms with a disease incidence of 10 per cent. The association of phytoplasma with the niger phyllody samples was confirmed by PCR using 16S rRNA, SecY and ribosomal protein (rp) gene-specific primers. The amplified PCR products were cloned and sequenced. The 16S rRNA, SecY and rp genes sequences analysis showed that niger phyllody (NiPP) phytoplasma shared a maximum nucleotide identity of 96.10 to 99.80 per cent (16S rDNA), 93.40 to 98.60 per cent (SecY gene) and 93.30 to 95.50 per cent (rp gene) with ‘Candidatus Phytoplasma asteris’ group (16SrI-D) isolates reported from different countries across the world. This was well supported by close clustering of NiPP phytoplasma with ‘Ca. P. asteris’ phytoplasmas belonging to the 16SrI-D group in the phylogenetic analysis. Further, the virtual RFLP pattern generated for the NiPP phytoplasma showed a similarity coefficient of 1.0 with ‘Ca. P. asteris’, 16SrI enclosed in 16Sr group I and subgroup D. As per our knowledge this is first report of ‘Ca. P. asteris’ belonging to 16Sr I-D group association with phyllody disease of niger in India. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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