Genotypic characterization of Plasmodiophora brassicae in the paddy-field weed Cardamine occulta and symptomology reveal a distinct pathogen population in Japan.

Autor: Lam, Anh Tung Phan, Sasaki, Kazunori, Yanagi, Yukiko, Tanaka, Shuhei, Ito, Shin-ichi
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Zdroj: Journal of General Plant Pathology; Jan2023, Vol. 89 Issue 1, p1-11, 11p
Abstrakt: Cruciferous clubroot, caused by the obligate protist Plasmodiophora brassicae, is one of the most destructive diseases of Brassicaceae crops worldwide. Clubroot is also frequently diagnosed in Cardamine occulta, a cruciferous weed in postharvest paddy fields across Japan, but the symptoms differ from those on the crop hosts. Galls were frequently detected in adventitious buds and even aerial parts such as petioles at several growth stages of the weeds. Phylogenetic analysis of concatenated actin gene and internal transcribed spacer 1 (ITS1) and 2 (ITS 2) revealed more variations and mutations (single-nucleotide polymorphisms, oligonucleotide polymorphisms, and insertions/deletions) in this novel population than in any published sequences for cosmopolitan P. brassicae strains from crops including other Japanese field populations (geographic isolates) collected here. Analysis of the ITS2 secondary structure for this newly discovered population provides new insights into diversity and phylogenetic relationships among P. brassicae populations, conclusively confirming its distinctive structure among the universal modeled structure for P. brassicae. Thus, the coevolution of the host weeds with P. brassicae isolates that are highly adapted to rice-paddy environments may significantly contribute to the evolution of P. brassicae on Brassica crops and club root epidemiology and needs further study. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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