Ribonucleic Acid Antigen from the Reiter Treponeme Used in an ELISA for Antibodies in Syphilis.

Autor: Pedersen, N. Strandberg, Petersen, C. Sand, Axelsen, N. H.
Předmět:
Zdroj: Scandinavian Journal of Immunology; Nov1982, Vol. 16 Issue 5, p431-436, 6p
Abstrakt: Purified RNA from Treponema phagedenis biotype Reiter was used as antigen in an enzyme-linked immunosorbent assay (ELISA) for IgG antibodies in syphilis. The RNA ELISA was compared with the fluorescent treponemal antibody absorption test (FTA-ABS), the Treponema pallidtum immobilization test (TP1), and with two ELISAs using puriliciJ flagelliim from the Reiter treponeme as antigen (flagellum ELISA), and sonified Reiter treponeme culture as antigen (sonicate ELISA). A total of 729 sera from patients with and without syphilis were studied. The RNA ELISA had a lower sensitivity (P < 0.01) in primary syphilis than the flagellum ELISA. the sonicate ELISA, and the ETA-ABS. In treated syphilis the RNA ELISA was also less sensitive than the TPI ( P < 0.01). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index