| Autor: |
Navarro-Ruiz, M. Carmen, Soler-Vázquez, M. Carmen, Díaz-Ruiz, Alberto, Peinado, Juan R., Nieto Calonge, Andrea, Sánchez-Ceinos, Julia, Tercero-Alcázar, Carmen, López-Alcalá, Jaime, Rangel-Zuñiga, Oriol A., Membrives, Antonio, López-Miranda, José, Malagón, María M., Guzmán-Ruiz, Rocío |
| Předmět: |
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| Zdroj: |
Biomedicines; Dec2022, Vol. 10 Issue 12, p3032, 20p |
| Abstrakt: |
Background: Obesity is characterized by adipose tissue dysregulation and predisposes individuals to insulin resistance and type 2 diabetes. At the molecular level, adipocyte dysfunction has been linked to obesity-triggered oxidative stress and protein carbonylation, considering protein carbonylation as a link between oxidative stress and metabolic dysfunction. The identification of specific carbonylated proteins in adipose tissue could provide novel biomarkers of oxidative damage related to metabolic status (i.e prediabetes). Thus, we aimed at characterizing the subcutaneous and omental human adipose tissue carbonylome in obesity-associated insulin resistance. Methods: 2D-PAGE was used to identify carbonylated proteins, and clinical correlations studies and molecular biology approaches including intracellular trafficking, reactive oxygen species assay, and iron content were performed using in vitro models of insulin resistance. Results: The carbonylome of human adipose tissue included common (serotransferrin, vimentin, actin, and annexin A2) and depot-specific (carbonic anhydrase and α-crystallin B in the subcutaneous depot; and α-1-antitrypsin and tubulin in the omental depot) differences that point out the complexity of oxidative stress at the metabolic level, highlighting changes in carbonylated transferrin expression. Posterior studies using in vitro prediabetic model evidence alteration in transferrin receptor translocation, linked to the prediabetic environment. Finally, ligand-receptor molecular docking studies showed a reduced affinity for carbonylated transferrin binding to its receptor compared to wild-type transferrin, emphasizing the role of transferrin carbonylation in the link between oxidative stress and metabolic dysfunction. Conclusions: The adipose tissue carbonylome contributes to understanding the molecular mechanism driving adipocyte dysfunction and identifies possible adipose tissue carbonylated targets in obesity-associated insulin resistance. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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