Enzymatic biotransformation of Rb3 from the leaves of Panax notoginseng to ginsenoside rd by a recombinant β-xylosidase from Thermoascus aurantiacus.

Autor: Zhang, Hui, Zhu, Hongxi, Luo, Xiuyuan, Deng, Yuanzhen, Zhang, Wei, Li, Shubo, Liang, Jingjuan, Pang, Zongwen
Předmět:
Zdroj: World Journal of Microbiology & Biotechnology; Jan2023, Vol. 39 Issue 1, p1-10, 10p
Abstrakt: Given the important pharmacological activity of ginsenoside Rd but its low content in plants, the production of Rd by enzymatic transformation is of interest. In this study, a β-xylosidase gene Ta-XylQS from Thermoascus aurantiacus was cloned and overexpressed in Komagataella phaffii. Purified recombinant Ta-XylQS specifically hydrolyzes substrates with xylosyl residues at the optimal pH of 3.5 and temperature of 60 °C. This study established a process for producing Rd by transforming ginsenoside Rb3 in the saponins of Panax notoginseng leaves via recombinant Ta-XylQS. After 60 h, 3 g L− 1 of Rb3 was transformed into 1.46 g L− 1 of Rd, and the maximum yield of Rd reached 4.31 g kg− 1 of Panax notoginseng leaves. This study is the first report of the biotransformation of ginsenoside Rb3 to Rd via a β-xylosidase, and the established process could potentially be adopted for the commercial production of Rd from Rb3. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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