| Autor: |
Bhattacharya, Sonali, Halder, Madhusree, Banerjee, Ankur, Das, Indrajit, Kundu, Surekha, Mandal, Deba Prasad, Bhattacharjee, Shamee |
| Zdroj: |
Proceedings of the Zoological Society; Dec2022, Vol. 75 Issue 4, p418-427, 10p |
| Abstrakt: |
Doxorubicin (Dox) is a potent and widely used chemotherapeutic drug. However, it has poor tissue penetration contributing to a low therapeutic index which severely limits the effectiveness of this anticancer drug. Nanoparticle-based delivery of Dox is a major approach to enhance its tissue penetration leading to enhanced efficacy at lower doses. In this study, platinum nanoparticles (PtNP) synthesized from the phytopathogenic fungus Curvularia affinis (CaPtNP), was used in combination with Dox in vitro in sarcoma-180 cells (S-180) and in vivo in S-180 transplantable tumor-bearing Swiss albino mice. IC 10, 20 and 30 concentrations of Dox and CaPtNP alone and in various combinations were used to treat S180 cells in vitro. Cell count and cell cycle study depicted more effective results in presence of increasing CaPtNP concentration with Dox. Moreover, fluorescence images and flowcytometric measurements showed increased Dox fluorescence in the combination treatment groups signifying enhanced cellular uptake of Dox in presence of CaPtNP. A similar trend ensued in vivo in tumor-bearing mice with a significantly greater reduction of tumor burden in the combination group than other groups. The enhanced anticancer effect was accompanied by an improved hepato-, myelo- and cardiotoxicity profile in the Dox + CaPtNP group. This study provides an enticing platform to develop Dox carrier which would reduce toxicity without compromising its anticancer property. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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