| Autor: |
Nautiyal, Aparna, Rashid, A., Agnihotri, Ashish |
| Předmět: |
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| Zdroj: |
In Vitro Cellular & Developmental Biology Plant; Dec2022, Vol. 58 Issue 6, p1126-1137, 12p |
| Abstrakt: |
The impact of different concentrations of thidiazuron (TDZ) and 6-benzylaminopurine (BAP) and the role of decapitation, flooding, and Ethrel® treatments were evaluated on direct in vitro caryopsis culture of rice. After 28 d of culture, multiple-shoot formation was observed from 50 to 90% of TDZ-treated seedlings. A similar but lower frequency response was observed using BAP-supplemented media. Furthermore, multiple shoots appeared to arise from the mesocotyl region of the seedlings. In subsequent experiments, isolation and transfer of mesocotyl segments to TDZ-supplemented media resulted in increased number of multiple shoots (10 to 12) as compared to intact seedlings (5 to 6). The formation of multiple shoots per seedling (13 to 15) and percent responding cultures with multiple shoots (70%) increased when decapitated rice seedlings were used instead of intact seedlings or mesocotyl segments. This demonstrated a possible role of physical stress in multiple-shoot formation along with other factors. Multiple shoots per seedling and percent responding cultures with multiple shoots also increased when intact seedlings were flooded with TDZ-supplemented media; however, flooding with distilled water and N6 had a very minor effect. Furthermore, the addition of Ethrel® to the culture media, which is metabolized within plant tissue to release ethylene, also resulted in multiple-shoot formation from seedlings. Based on these multiple lines of evidence, this study proposes that TDZ is acting to enhance multiple-shoot formation in this rice system through activation of stress-related gene(s) and signaling molecules. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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