| Autor: |
Chen, Qiang, An, Yuchao, Feng, Mingjian, Li, Jincheng, Li, Yanjie, Tong, Feifei, Qu, Ge, Sun, Zhoutong, Wang, Yujun, Luo, Guangsheng |
| Předmět: |
|
| Zdroj: |
Green Chemistry; 12/21/2022, Vol. 24 Issue 24, p9508-9518, 11p |
| Abstrakt: |
Green, efficient, and sustainable flow synthesis of intermediate chiral aryl alcohols is critical for continuous drug-manufacturing. Enzyme immobilization endows flow biocatalysis with higher application potential; however, conventional methods are limited by cumbersome operations, a trade-off between activity and stability, and low loading due to the required support. Here, for the first time, we demonstrated the microfluidic induction of alcohol dehydrogenase to form enzyme-assembled gels (EAGs) in a microreactor, with controllable mechanical properties and a porous architecture. Six chiral aryl alcohols were successfully synthesized by this approach in flow asymmetric reduction. Moreover, the support-free EAG was capable of in situ flow immobilization and reversible de-immobilization without reactor disassembly. The EAG monolithic microreactor presented an excellent space–time yield of 88.05 g L−1 h−1 and a high total turnover number of 62 600 after 100 h of operation (over 1300 reactor volumes), highlighting its high activity and stability. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
