| Autor: |
Barmukh, Rutwik, Roorkiwal, Manish, Dixit, Girish P, Bajaj, Prasad, Kholova, Jana, Smith, Millicent R, Chitikineni, Annapurna, Bharadwaj, Chellapilla, Sreeman, Sheshshayee M, Rathore, Abhishek, Tripathi, Shailesh, Yasin, Mohammad, Vijayakumar, Adiveppa G, Sagurthi, Someswar Rao, Siddique, Kadambot H M, Varshney, Rajeev K |
| Předmět: |
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| Zdroj: |
Journal of Experimental Botany; 12/8/2022, Vol. 73 Issue 22, p7255-7272, 18p |
| Abstrakt: |
' QTL-hotspot ' is a genomic region on linkage group 04 (CaLG04) in chickpea (Cicer arietinum) that harbours major-effect quantitative trait loci (QTLs) for multiple drought-adaptive traits, and it therefore represents a promising target for improving drought adaptation. To investigate the mechanisms underpinning the positive effects of ' QTL-hotspot ' on seed yield under drought, we introgressed this region from the ICC 4958 genotype into five elite chickpea cultivars. The resulting introgression lines (ILs) and their parents were evaluated in multi-location field trials and semi-controlled conditions. The results showed that the ' QTL-hotspot ' region improved seed yield under rainfed conditions by increasing seed weight, reducing the time to flowering, regulating traits related to canopy growth and early vigour, and enhancing transpiration efficiency. Whole-genome sequencing data analysis of the ILs and parents revealed four genes underlying the ' QTL-hotspot ' region associated with drought adaptation. We validated diagnostic KASP markers closely linked to these genes using the ILs and their parents for future deployment in chickpea breeding programs. The CaTIFY4b-H2 haplotype of a potential candidate gene CaTIFY4b was identified as the superior haplotype for 100-seed weight. The candidate genes and superior haplotypes identified in this study have the potential to serve as direct targets for genetic manipulation and selection for chickpea improvement. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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