Evaluation of novel surfactants for plant transformation.

Autor:	Huynh, Jennie, Hotton, Sara K., Chan, Ron, Syed, Yasra, Thomson, James
Předmět:	PLANT genetic transformation SURFACE active agents ARABIDOPSIS
Zdroj:	BMC Research Notes; 12/8/2022, Vol. 15 Issue 1, p1-5, 5p
Abstrakt:	Objective: Assess the efficiency of seven novel surfactants, relative to the typical Silwet® L-77, for floral dip transformation of Arabidopsis. Results: Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare seven novel surfactants (BREAK-THRU®-OE446, S200, S233, S240, S279, S301 and SP133) to the standard Silwet® L-77 for efficiency of Arabidopsis transformation providing an example of how the surfactants can help other plant transformation protocols. Relative transformation efficiencies ranged from − 44 to + 45% compared to Silwet® L-77. Surfactants S200, S240, and S279 demonstrated the greatest enhancement in transformation. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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