Evaluation of antibacterial activity of ultrasound-assisted hydrodistillation of Amomum cardamomum oil.

Autor: Ekapratiwi, Yasmin, Rachmawati, Ade, Agustian, Egi, Dewi, Rizna Triana, Sulaswatty, Anny
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Zdroj: AIP Conference Proceedings; 12/5/2022, Vol. 2493 Issue 1, p1-5, 5p
Abstrakt: This study evaluates the antibacterial activity of Amomum cardamomum oil extracted with ultrasound-assisted hydrodistillation (UAHD), compared to conventional hydrodistillation (HD). HD and UAHD were carried out by Clevenger-type apparatus with SFR 1:20 for 6 h. UAHD was conducted in various energy outputs (30%, 60%, and 90%) as pre-treatment. The results showed that the power used by UAHD increases oil volume with the highest yield is 6.97% using 90% of energy. The chemical composition analysis was determined by GC-MS and showed that 1,8 cineole dominates up to 60% of total oil compositions. Antibacterial activity was determined by zone of inhibition (ZOI) using disk diffusion and minimum inhibition concentration (MIC) using microdilution method against Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. The ZOI ranged between 9±0.0-14±2.0 mm and the MIC valued 1.25 and 2.5 mg/mL against all tested bacteria. However, based on Kruskal-Wallis analysis, there was no marked difference in antibacterial activity of both oils extracted with UAHD and HD, which they possessed similar ZOI and MIC. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index