| Autor: |
Ebrahimzadeh, Mohammad Hadi, Naseri, Mahboobeh, Younesikelaki, Fatemeh Soghra, Javan, Iman Yousefi, Swamy, Rama N. |
| Předmět: |
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| Zdroj: |
International Journal of Horticultural Science & Technology; Sep2023, Vol. 10 Issue 3, p223-235, 13p |
| Abstrakt: |
Silybum marianum (L.) is a medicinally important herb known for its liver-protecting compound, silymarin, which is an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol was designed for the application of plant growth regulators at various concentrations and combinations of coconut water. This was aimed at initiating callus and silymarin production using different types of explants (root, hypoc -otyls, cotyledonary leaf) of S. marianum. Free radical scavenging activity of silymarin was evaluated in the extracts of callus cultures of these explants. In comparison with other plant growth regulators and explants, root explants that were cultured on MS medium and were enriched with 5.0 M NAA + 2.5 M BAP + 10% coconut water produced the most friable callus and had the highest possible level of silymarin and free radical scavenging activity. NAA + BAP + coconut water was found to be the best hormonal combination for callus initiation and mass production. This research can aid in the current understanding of the influence of plant growth regulators and different types of explants on the production of valuable secondary metabolites in S. marianum via in vitro cultures. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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