| Autor: |
Vasconcelos, Mariana Galvão Gurgel Cabral de, Jarovsky, Daniel, Nunes, Gabriela Zembruski, Tridente, Daniela Marinho, Grill, Juliana Amorim Teixeira, Berezin, Eitan Naaman, de Vasconcelos, Mariana Galvão Gurgel Cabral |
| Předmět: |
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| Zdroj: |
Journal of Tropical Pediatrics; Dec2022, Vol. 68 Issue 6, p1-6, 6p |
| Abstrakt: |
Background: The etiological diagnosis of community-acquired pneumonia (CAP) is still a challenge. We compared the conventional culture method and real-time polymerase chain reaction (RT-PCR) for the identification of Streptococcus pneumoniae in severe pediatric CAP.Methods: A retrospective hospital-based study was conducted. From 2012 to 2018, we have selected patients who had peripheral blood and/or pleural fluid collected for etiological investigation by RT-PCR.Results: We included 113 children (median age: 3 years; interquartile range 1-6 years). RT-PCR increased the detection rate of S. pneumoniae by 6.5 times using blood samples and eight times using pleural fluid samples. Patients subjected to RT-PCR showed more prolonged hospitalization (p = 0.006), fewer comorbidities (p = 0.03), presence of pleural effusion (p = 0.001), presence of young forms of leukocytes (p = 0.001) and radiograph with characteristics of pneumonia (p = 0.002). The presence of pleural effusion [odds ratio (OR) = 14.7, 95% confidence interval (CI) 1.6-133.9; p = 0.01] and young forms of leukocytes (OR = 8.9, 95% CI 0.9-84.4; p = 0.05) were risk factors for positive RT-PCR pneumococcal when multivariate analysis was performed.Conclusions: RT-PCR is a reliable method for diagnosing severe CAP using sterile materials and a potentially applicable method in patients with clinical, radiological and non-specific laboratory characteristics of lower respiratory tract infection, especially in complicated cases with pleural effusion. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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