| Autor: |
Вълчков, Александър, Марков, Злати, Логиновска, Камелия, Солаков, Николай, Донева, Мария |
| Předmět: |
|
| Zdroj: |
Journal of Mountain Agriculture on the Balkans (JMAB); 2022, Vol. 25 Issue 4, p286-303, 18p |
| Abstrakt: |
Chitinases are effective agents for biological control of plant diseases caused by various phytopathogenic fungi. This determines the significant biotechnological potential for the production of chitinase enzymes and their use as biological agents for plant disease control. Experienced experiments were performed using six different nutrient media to cultivate a producer strain of Bacillus subtilis NBIMCC 2353 in order to increase the enzyme chitinase produced and increase the viable cell number. Successful separation of the culture fluids from the studied media was achieved by the methods of separation − centrifugation and filtration. Clear solutions with enzymatic activity (supernatants) and cloudy solutions (precipitates) containing the cells of the producer strain were obtained. The supernatants obtained by the filtration method were found to have higher enzymatic activity than those obtained by centrifugation. Similarly, the precipitates obtained by the filtration method have a larger number of cells than those obtained by the centrifugation method. In both methods of separation with the highest chitinase activity, are distinguished the supernatants from the modified nutrient medium with added fresh mycelium of Ganoderma lucidum, and with the highest number of viable cells − the sediments from the same medium. High values for chitinase activity and viable cell count were also obtained in supernatants and sediments from the nutrient media with added chitin and arabinose in both methods of separation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
