| Autor: |
Masahiko Oshige, Ryo Yokosawa, Hitomi Shinohara, Maho Suzuki, Shinji Katsura |
| Předmět: |
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| Zdroj: |
International Journal of Plasma Environmental Science & Technology (IJPEST); 2022, Vol. 16 Issue 3, p1-9, 9p |
| Abstrakt: |
The reaction temperature plays a key role in observing biochemical reactions through a microscope. In conventional methods, the actual local temperature of the observed area is different from the measured local temperature because the heater and the temperature sensor are far apart. The electrical resistance of indium tin oxide, a transparent electrode, changes with its temperature. Based on this property, we developed a temperature control system for the viewing area of a microscope. In this system, we heated the transparent electrode by Joule heating, and temperature measurement is conducted on the same electrode in a time-sharing manner. Then, we applied this system to temperature cycles for a reverse transcription-polymerase chain reaction in emulsions. Successful DNA amplification was confirmed by observing the fluorescence of FAM, whose quenching was terminated via oligonucleotide cleavage by DNA amplification in the emulsions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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