| Abstrakt: |
In this work, an amperometric biosensor for ALT detection, which will be part of a biosensor system for simultaneous measurements of AST and ALT and their ratios, has been developed. A platinum disk electrode was used as the electrochemical transducer. To form a bioselective element, a mixture based on glutamate oxidase was applied to the surface of transducer. A universal method for measuring ALT activity using the developed biosensor was proposed and described. During the study, the optimal concentrations of ALT substrates (α-ketoglutarate – 50 μM, alanine – 4 mM) and coenzyme (50 μM of pyridoxal phosphate) were selected. The analytical characteristics of the developed biosensor were also investigated, namely, sensitivity, linear range, minimum limit of determination, selectivity, stability, etc. It was shown the fundamental possibility of using this biosensor to measure the content of ALT in real samples. [ABSTRACT FROM AUTHOR] |
