| Autor: |
Yifan Wang, Ong, Jasmine, Oi Wing Ng, Tapanut Songkasupa, Koh, Eileen Y., Wong, Jeslyn P. S., Kanokwan Puangjinda, Fernandez, Charlene Judith, Taoqi Huangfu, Lee Ching Ng, Siow Foong Chang, Him Hoo Yap, Wang, Yifan, Ng, Oi Wing, Songkasupa, Tapanut, Puangjinda, Kanokwan, Huangfu, Taoqi, Ng, Lee Ching, Chang, Siow Foong, Yap, Him Hoo |
| Zdroj: |
Emerging Infectious Diseases; Dec2022, Vol. 28 Issue 12, p2446-2454, 9p, 2 Diagrams, 4 Charts |
| Abstrakt: |
African horse sickness (AHS) is a highly infectious and often fatal disease caused by 9 serotypes of the orbivirus African horse sickness virus (AHSV). In March 2020, an AHS outbreak was reported in Thailand in which AHSV serotype 1 was identified as the causative agent. Trivalent live attenuated vaccines serotype 1, 3, and 4 were used in a targeted vaccination campaign within a 50-km radius surrounding the infected cases, which promptly controlled the spread of the disease. However, AHS-like symptoms in vaccinated horses required laboratory diagnostic methods to differentiate infected horses from vaccinated horses, especially for postvaccination surveillance. We describe a real-time reverse transcription PCR-based assay for rapid characterization of the affecting field strain. The development and validation of this assay should imbue confidence in differentiating AHS-vaccinated horses from nonvaccinated horses. This method should be applied to determining the epidemiology of AHSV in future outbreaks. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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