| Autor: |
Katoch, Kajal, Gupta, Suphla, Dey, Abhijit, Pandey, Devendra Kumar |
| Zdroj: |
Nucleus (0029568X); Dec2022, Vol. 65 Issue 3, p351-358, 8p |
| Abstrakt: |
Plumbago auriculata Lam. (Plumbaginaceae) is a medicinal flowering shrub which produces plumbagin that shows diverse pharmacological attributes. P auriculata accumulates a very important napthaquinone, plumbagin which exhibits a wide range of medicinal properties. The present study is aimed at in vitro propagation of P auriculata and a comparative quantification of plumbagin in in vitro and in vivo grown plants. Direct shoot regeneration was induced from the nodal explants of P auriculata. Murashige and Skoog medium supplemented with different combination of 6-Benzylaminopurine (BAP) and 1-Naphthaleneacetic acid (NAA). Best response was obtained with BAP 1.5 mg/L and NAA 2.0 mg/L after 15 days in in vitro culture. Maximum number of roots were induced on ½ strength MS medium supplemented with 2% sucrose (w/v) and 0.7 mg/L Indole-3-acetic acid. Plumbagin was extracted by microwave-assisted extraction method and content was estimated from stem, leaf and root of both propagated plants by HPTLC. It was found that the roots of both in vivo (0.01% w/w) and in vitro (0.066% w/w) grown plants contain the highest amount of plumbagin. In vitro grown roots produced five-fold more plumbagin as compared to in vivo regenerated roots. Maximum increase of plumbagin production was observed in the in vitro grown stem i.e., seven-fold as compared to in vivo induced stem. In vitro propagation of plant provides a rapid and continuous supply of plumbagin while preserving the natural resource. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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