| Autor: |
Müller, Jan, Ott, Vivian, Weiss, Noémi, Neubauer, Peter, Eibl, Dieter, Eibl, Regine |
| Předmět: |
|
| Zdroj: |
Chemie Ingenieur Technik (CIT); Dec2022, Vol. 94 Issue 12, p1977-1984, 8p |
| Abstrakt: |
Various approaches to process intensification are currently being investigated to ensure time and cost savings when producing biopharmaceuticals. In the present study, a one‐step inoculum production was established based on an ultra‐high cell density working cell bank with immunoglobulin G producing Chinese hamster ovary cells. Cryovials were used for direct inoculation of 1‐L wave‐mixed perfusion bioreactors. When reaching around 180 · 106 cells mL−1 in N−1 perfusion mode, low‐seed and high‐seed fed‐batch experiments in shake flasks were inoculated, as was the case for 250‐mL stirred single‐use bioreactors. Additionally, proof‐of‐concept runs at 50‐L and 200‐L scale were successfully performed. The intensification approach presented allows manufacturing capacity to be increased by up to 50 %. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
