| Abstrakt: |
Polymeric and monomeric human IgA were isolated from the sera of patients with IgA myeloma; rat IgA polymer, monomer and 1gG2 were isolated from the ascitic fluid or sera of Lou/Wsl rats bearing appropriate myelomata. The purified Ig preparations were labelled with 125I and injected intravenously into rats, rabbits, guinea-pigs or sheep that had had a cannula inserted into the common bile duct so that their bile could be collected quantitatively. Rats and rabbits transported 30% of the injected dose of both IgA polymers, but no other type of immunoglobulin, from blood to bile within 5-1 h. Sheep and guinea-pigs were unable to transport any of the immunoglobulin preparations from blood to bile, even though the injected material remained circulating in the blood. Lemaître-Coelho, Vaerman, Bazin & Beckers, 1978) by the hepatocytes (Birbeck, Cartwright, Hall, Orlans & Peppard, 1979). Like enterocytes, the hepatocytes have SC on their surfaces and apparently transport IgA in much the same way (Orlans, Peppard, Fry, Hinton & Mullock, 1979; Socken, Jeejeebhoy, Bazin & Underdown, 1979; Mullock, Hinton, Dobrota, Peppaul & Orlans, 1979). Since it is relatively easy to study the extent and kinetics of IgA transport by collecting bile, and since there is evidence that IgA and SC from different species will interact in vitro (Mach, 1970; Socken & Underdown, 1978) we have used this method to study the transport of heterologous IgA in four mammalian species. [ABSTRACT FROM AUTHOR] |
