"OUTCOME OF ANAEROBIC CULTURE IN POSTOPERATIVE OSTEOMYELITIS CASES USING ROBERTSON'S COOKED MEAT BROTH-A PROSPECTIVE OBSERVATIONAL STUDY".

Autor: Mathapati, Shrikanth, M. S., Shashi Kumar, M. U., Abhishek
Předmět:
Zdroj: Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research); 2022, Vol. 13 Issue 7, p620-629, 10p
Abstrakt: INTRODUCTION: Osteomyelitis remains as a disastrous outcome postoperatively which is associated with increased morbidity and high economic burden. Although bacterial oraganisms have been identified as the major causative agents, role of anaerobic bacteria is discussed infrequently. Understanding the occurrence, type of osteomyelitis and its causative agents helps in treating osteomyelitis effectively. OBJECTIVES: to analyze the occurrence of different types of osteomyelitis and the contributing factors to the condition. It mainly emphasis on screening the postoperative osteomyelitis specimen for anaerobic bacteria by culture using Robertson's Cooked Meat Broth (RCMB) medium and interpreting its outcome to aid in treatment. METHODS: This prospective observational study was conducted for the period of 2 years from august 2018 to September 2020 in department of orthopaedics at AJ shetty institute of medical sciences, mangalore. 50 Patients with osteomyelitis occurring post fracture fixation or wound debridement were included in this study after obtaining informed consent. patients were evaluated clinically and radiologically. The infected specimens were processed for gram staining and anaerobic culture using robertson's cooked meat broth. Association between Variables was analyzed by using Chi-Square test for categorical Variables. Level of significance was set at 0.05. RESULTS: This hospital-based observational study included 50 patients with postoperative osteomyelitis. Majority (53.3%) presented with chronic osteomyelitis, with tibia being the most common bone involved(74%). Organisms were isolated via culture where aerobic bacteria were majority constituting 75.6% followed by 17.8% of facultative anaerobes. Only a single patient was found to have anaerobic infection in RCMB medium which was of bacteroides species. The Chi square value for type of osteomyelitis and type of infection was 31.177, p = 0.000, showing that there is statistically significant association between the two and depicting the occurrence of anaerobic infection in chronic osteomyelitis most commonly. CONCLUSION: Incidence of anaerobic infection in postoperative osteomyelitis remains less compared to aerobic organisms and Robertson's cooked meat broth medium remains an vital culture medium in isolating anaerobic organisms. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index