| Autor: |
Danis, V. A., Franic, G. M., Rathjen, D. A., Brooks, P. M. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Immunology; Jul1991, Vol. 85 Issue 1, p143-150, 8p |
| Abstrakt: |
The effects of GM-CSF, IL-2, IFN-γ, TNF-α and IL-6 on the production of IL-I (both secreted and ceil associated) and TNF-α by peripheral blood monocytes were studied. Monocytes were cultured for 20 h in suspension and in serum-free conditions which minimized background stimulation of monokine production. GM-CSF, IL-2 and TNF-α directly induced the production of cell-associated IL-1 but little or no IL-1 or TNF-α secretion. Combination of GM-CSF with IFN-γ, IL-2 or TNF-α synergistically enhanced IL-1 secretion and had an additive effect on cell-associated IL-l production. Combination of IL-2 with IFN-7 or TNF-α also synergistically enhanced IL-1 secretion but the effect on cell-associated IL-1 production was less than additive. GM-CSF synergistically enhanced TNF-α secretion induced by IFN-γ but not by lipopolysaccharide. GM-CSF did not enhance TNF-α secretion induced by IL-2 or TNF-α. In contrast. IL-2 synergistically enhanced TNF-α secretion induced by IFN-γ. These results are discussed in relation to cylokine involvement in rheumatoid arthritis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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