| Autor: |
Martyniuk, Viktoria, Gylytė, Brigita, Matskiv, Tetiana, Khoma, Vira, Tulaidan, Halyna, Gnatyshyna, Lesya, Orlova-Hudim, Kateryna, Manusadžianas, Levonas, Stoliar, Oksana |
| Předmět: |
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| Zdroj: |
Ecotoxicology; Nov2022, Vol. 31 Issue 9, p1369-1381, 13p |
| Abstrakt: |
Even though bivalve molluscs are recognized as bioindicators of freshwater quality, their responses to multiple stressors are unpredictable. This study aims to elucidate the inter-population peculiarities of the effect in the sub-chronic environmentally relevant exposure to novel contaminants. The specimens of Unio tumidus from reference (Pr) and contaminated (Ct) areas were treated with ibuprofen (IBU, 0.8 µg L−1), microplastic (MP, 1.0 mg L−1, size 0.1–0.5 mm), or their combination (Mix) for 14 days. Untreated mussels (PrC- and CtC-groups) served as controls. The PrC-group had higher levels of antioxidants Mn-SOD, Cu,Zn-SOD, catalase, and cholinesterase (AChE) as well as lesser levels of oxidative lesions (TBARS and protein carbonyls) in digestive glands, indicating lower environmental impact than in the CtC-group. However, lysosomal stability was similar in both control groups. Among antioxidants, Mn-SOD activity was affected most prominently, increasing in all exposed Ct-groups. TBARS level was increased only in PrMP-group compared to responsive control. IBU and Mix enhanced protein carbonyl concentration in the Pr-groups, and decreased it in the Ct-groups. AChE was induced in the CtIBU- and PrMix-groups, and lysosomal integrity increased in the CtIBU and CtMix-groups. Discriminant analyses indicated lesser differences between Pr-groups, demonstrating lower cumulative stress compared to Ct-groups. Generally, the most remarkable response was revealed in the CtIBU-group, and distortion of individual effects was established in combined exposures. The qualification of stress-neutral and stress-positive populations was proposed for Pr- and Ct-populations correspondingly. Inter-site peculiarities must be taken into consideration when the environmental impact of MP and pharmaceuticals is evaluated. Highlights: Molluscs from contaminated (Ct) and reference (Pr) sites are biochemically distinguishable. Ibuprofen enhanced antioxidant defense in the Ct-molluscs. Ibuprofen induced cholinesterase and lysosomal integrity in Ct-molluscs. Microplastic caused oxidative stress in Pr-molluscs only. Combine exposures distort the responses in the Pr- and Ct-molluscs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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