| Autor: |
Alsaffar, Dania F., Alsaffar, Sura F., Yaseen, Ahmed, Sulaiman, Shaida Fariza, Mahmud, Roziahanim, Abdul Aziz, Nur Hidayah Kaz |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Oct2022, Vol. 22 Issue 2, p4121-4132, 12p |
| Abstrakt: |
Euphorbia hirta L., belonging to the Euphorbiaceae family, is widely used in traditional remedies and have different pharmacological applications. Samples of the plant were collected in Malaysia and Iraq. The objectives of this study are to understand how variations in climate and geography might affect the plant's nutrient, secondary metabolite and antioxidant levels. The aerial part of Euphorbia hirta plant was simultaneously collected in Malaysia and Iraq. Hot and cold extraction methods involving the use of hexane, chloroform, methanol and water solvents were then undertaken. All extracts were subsequently screened for the presence of phytochemicals and total phenolic and flavonoid contents. In addition, in vitro antioxidant and anti-inflammatory activities were conducted. Finally, the optimum extracts (metabolic extracts) of the two species were subjected to LC-MS/MS analysis. The results showed that the highest phenolic and flavonoid content, antioxidant properties and anti-inflammatory activities were found in the methanolic extract of the Malaysian species obtained using hot methods (336.65±9.46 mg Gallic acid equivalent of dry plant material weight, 63.94±2.81mg (QE)/g dry wt. 80.80±0.53% and 88.81% respectively). An LC-MS/MS analysis of the two species revealed the presence of various phenolic, flavonoids and steroid compounds. It can be conclude that differences in agro-climatic conditions, extraction methods and types of extraction solvent used have clear effects on the total phenolic and flavonoid content, and the antioxidant and anti-inflammatory potential of the E. hirta plant. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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