| Autor: |
Sunari, I. Gusti Agung Ayu Eka Putri, Nugraha, Jusak |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Apr2022, Vol. 22 Issue 1, p1677-1680, 4p |
| Abstrakt: |
The role hepatitis B antibodies (Anti-HBs) is to assess immunity following HBV infection and find out whether there is protection after vaccination. Anti-HBs titers are protective if their levels are >10mIU/mL. Several methods of testing anti-HBs levels can produce varying results. This study used Electrochemiluminescence Immunoassay (ECLIA) and Chemiluminescence immunoassay (CLIA) methods to measure the levels of anti-HBs. This study is to determine the relationship between the ECLIA and CLIA methods in measuring anti-HBs levels. This was a cross sectional observative analytic study with purposive sampling. Samples were from patients who received the first vaccine or Hepatitis B booster in 2018-2019. Examination of anti-HBs levels was carried out using ECLIA and CLIA methods. Levels of anti-HBs were analyzed using Paired-t test with a confident interval (CI) 95%. Correlation test of both methods used Pearson test and Bland Altman. Only 30 samples were eligible to be tested, consisting of 28 females and 2 males. Vaccination history showed 50% of the first vaccine and 50% of booster. The mean anti-HBs levels in ECLIA was higher than CLIA. The paired-t test results were significant with a p value = 0.009 and Pearson test 0.83 showed positive and strong correlation. Both methods showed good relationship. There was one sample that provided protective result by ECLIA but not protective by CLIA. The difference in antibody levels between the two methods could be influenced by the presence of virus mutations, differences in type and avidity of the test antigen, differences in IgG subclasses to the time of administration of the Hepatitis B vaccine. ECLIA and CLIA methods had a good relationship in measuring anti-HBs levels. The mean of anti-HBs level by ECLIA was higher than CLIA. Both methods ware suitable for measuring anti-HBs levels. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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