A peptide corresponding to the neuropilin-1-binding site on VEGF(165) induces apoptosis of neuropilin-1-expressing breast tumour cells.
| Autor: | Barr, M. P., Byrne, A. M., Duffy, A. M., Condron, C. M., Devocelle, M., Harriott, P., Bouchier-Hayes, D. J., Harmey, J. H. |
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| Předmět: |
TUMORS
CANCER cells APOPTOSIS CELL death CYTOKINES TYROSINE ADENOCARCINOMA FLOW cytometry RESEARCH MICROSCOPY WESTERN immunoblotting ANIMAL experimentation RESEARCH methodology CELL receptors MEDICAL cooperation EVALUATION research COMPARATIVE studies CELL lines EPITHELIAL cells VASCULAR endothelial growth factors BREAST tumors PEPTIDES MICE |
| Zdroj: | British Journal of Cancer; 1/31/2005, Vol. 92 Issue 2, p328-333, 6p |
| Abstrakt: | There is increasing evidence that vascular endothelial growth factor (VEGF) has autocrine as well as paracrine functions in tumour biology. Vascular endothelial growth factor-mediated cell survival signalling occurs via the classical tyrosine kinase receptors Flt-1, KDR/Flk-1 and the more novel neuropilin (NP) receptors, NP-1 and NP-2. A 24-mer peptide, which binds to neuropilin-1, induced apoptosis of murine and human breast carcinoma cells, whereas a peptide directed against KDR had no effect. Both anti-NP1 and anti-KDR peptides induced endothelial cell apoptosis. Confocal microscopy using 5-(6)-carboxyfluorescein-labelled peptides showed that anti-NP1 bound to both tumour and endothelial cells, whereas anti-KDR bound endothelial cells only. This study demonstrates that NP-1 plays an essential role in autocrine antiapoptotic signalling by VEGF in tumour cells and that NP1-blockade induces tumour cell and endothelial cell apoptosis. Specific peptides can therefore be used to target both autocrine (tumour cells) and paracrine (endothelial cells) signalling by VEGF. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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