| Autor: |
de Albuquerque-Melo, Barbara Cristina, Cysne-Finkelstein, Léa, Gonçalves-Oliveira, Luiz Filipe, Cascabulho, Cynthia Machado, Henriques-Pons, Andrea, Pereira, Mirian Cláudia de Souza, Alves, Carlos Roberto, Dias-Lopes, Geovane |
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| Zdroj: |
Parasitology; Oct2022, Vol. 149 Issue 12, p1526-1535, 10p |
| Abstrakt: |
Keywords: CFSE labelling; cytokines; in vitro infection; Leishmania (Viannia) braziliensis; murine peritoneal macrophages; population structure EN CFSE labelling cytokines in vitro infection Leishmania (Viannia) braziliensis murine peritoneal macrophages population structure 1526 1535 10 11/08/22 20221001 NES 221001 Introduction I Leishmania (Viannia) braziliensis i was the first I Leishmania i species assigned as the aetiological agent of American tegumentary leishmaniasis (ATL), (Lainson, 1983; Grimaldi I et al i ., 1987). The Thor10/Thor22 combinations significantly downregulated the TNF I i levels at both 24 and 48 h of infection, contrasting to this subpopulation alone and its Thor10/Thor22 (1:3) combination at 48 h [4.4 pg mL SP -1 sp in Thor10 and 4.2 in Thor10/Thor22 (1:3)] (Fig. Infection of peritoneal macrophages The macrophage infection assays were performed with the stationary phase of promastigotes at a ratio of 10:1 (parasite:macrophage) with 2 h or 5 h interaction (37°C in 5% CO SB 2 sb ). Notably, the macrophage infection rate (%) was strongly influenced by different initial interaction times, with a tendency to increase at 5 h, in combinations with 50% and 75% of the Thor10 and Thor03 [Thor10/Thor22(1:1 and 3:1); Thor03/Thor22(1:1 and 3:1)] as well as 25% of Thor10 [10/22(1:3)], which reached 45% and 85% of infection at 2 h and 5 h, respectively (Fig. In general, the subpopulations with higher virulence (Thor03 and Thor10) increased the macrophage infection rate when in combination with Thor22, except for Thor03/Thor22 (1:3). [Extracted from the article] |
| Databáze: |
Complementary Index |
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