PHENOTYPIC DETECTION OF EXTENDED SPECTRUM β LACTAMASE PRODUCING ESCHERICHIA COLI ISOLATES FROM DIARRHOEIC LAMBS.

Autor: Sujatha, T., Srivani, M., Subramanyam, K. V., Srinivasa Rao, T.
Předmět:
Zdroj: Journal of Experimental Zoology India; Jul2022, Vol. 25 Issue 2, p1323-1327, 5p
Abstrakt: A study was carried out to find out the distribution of Extended-spectrum â lactamase (ESBL) producing Escherichia coli isolates from diarrhoeic lambs in Andhra Pradesh. A total of 212 rectal swabs were collected from 1 to 90-dayold diarrhoeic lambs from Vizianagaram, West Godavari, and Krishna districts of Andhra Pradesh of which 170 (80.18%) E. coli were isolated based on cultural, biochemical, and molecular characterization. Among the E. coli isolates 140 (87.05%) were Shiga toxin-producing E. coli (STEC). An ESBL phenotype was confirmed in a total of 72 STEC isolates of which 51.38% (37/72) of isolates showed ESBL production when tested with CCL combination discs whereas 51.38% (37/72) and 48.61% (35/72) of the isolates exhibited ESBL production when tested with CEC and CAC combination discs, respectively. DDST test revealed Only 47.79 isolates were ESBL producers. This study concluded that CCL and CEC combination discs were found more sensitive in the detection of ESBL in STEC. Further, these methods in conjunction with antibiotic sensitivity tests will be useful in formulating therapeutic strategies to control lamb diarrhoea. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index