EFFECT OF SYNBIOTIC ON INTESTINAL HISTOMORPHOMETRY AND GROWTH RATE IN QUAILS, EXPERIMENTALLY INFECTED WITH FIELD STRAIN OF SALMONELLA GALLINARUM.

Autor: Khan, A. A., Aslam, A., Rehman, M., Shehzad, W., Abbas, G., Tanveer, A. J., Raffie, A. R., Ayub, Z.
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Zdroj: Pakistan Journal of Science; 6/30/2022, Vol. 74 Issue 2, p101-101, 1p
Abstrakt: ABSTRACT This study was planned to investigate the effect of synbiotics on intestinal histomorphometry and the growth rate of quails experimentally infected with the field strain of Salmonella gallinarum. Day-old Japanese quails (120), with an average body weight of 6.09±1.1 g, were randomly assigned into 4 groups AA, BB, CC, and DD, and 2 replicates. Quails of group AA and BB were given synbiotics on a daily and weekly basis respectively, along with challenge while group CC was the negative control and group DD was the positive control group. A total of 60 organ samples of the intestine were collected from apparently healthy and freshly dead quails respectively to isolate the field strain of Salmonella gallinarum. Bacterial isolation and molecular identification were performed by laboratory diagnostic culture techniques and PCR. Results from the current study indicate that non-antibiotic feed additives such as synbiotics used in this study boosted the gut histomorphometric parameters including the villus height, villus width, and crypt depth under the challenge of Salmonella gallinarum. There was a significant increase in all these parameters due to synbiotic feeding except the negative control group which showed the lowest values. At day 21, DD achieved a maximum villus height of 636.88 ± 65.93b µm in the duodenal mucosa, whereas, maximum villus width of 182.01 ± 15.40c µm in duodenal mucosa was achieved by group AA. Maximum villus height of 276.89 ± 21.16b µm and crypt depth of 26.66 ± 1.15a µm in jejunum mucosa were recorded in group AA. Hence, statistical results using the technique of one-way ANOVAs indicated that there was a significant increase in morphometric parameters of duodenal and jejunum mucosa in the groups fed synbiotics as compared to the negative control. Hence results illustrate that there is an overall increase in histological parameters of duodenal and jejunum mucosa in the groups fed synbiotics as compared with the negative control group. This study supports the beneficial effects of synbiotics on intestinal health and the growth rate of Japanese quails by improving FCR. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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