| Autor: |
Zavazava, N., Halene, M., Westphal, E., Nölle, B., Duncker, G., Eckstein, E., Harpprecht, J., Müller-Ruchholtz, W. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Immunology; Apr1991, Vol. 84 Issue 1, p163-166, 4p |
| Abstrakt: |
The objective of this study was to investigate the expression of MHC antigens by retinal pigment epithelium cells ( RPE) after stimulation with interferon-gamma (IFN-γ) and to improve the currently practised technique of cadaver HLA typing. A concentration of 100 U/ml IFN-γ induced expression of class I molecules up to > 90% 3 days after stimulation, whereas 50 U/ml were required for the expression of HLA-DR to >90%. A concentration of 750 U/ml induced 35-45% expression of HLA- DP and <25% HLA-DQ after 3 days. Cells were serologically typed using the standard lymphocytotoxicity assay 3 days after stimulation with 250 U/ml IFN-γ. Typing of class I specificities was complemented by one-dimensional isoelectric focusing (1 D-IEF). We observed high concordance between the results of the RPE typing and the lymphocytotoxicity test on the same donors. Our results show complete typing of class I and II antigens post-mortem, which, in particular, enables graft matching and improvement of graft survival in recipients of organs removed many hours after death such as the cornea. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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