| Autor: |
Kelly, C. D., Russo, C. M., Rubin, B. Y., Murray, H. W. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Immunology; Sep1989, Vol. 77 Issue 3, p397-402, 6p |
| Abstrakt: |
In response to cytomegalovirus (CMV) and Toxoplasma gondii antigens, T4+ cells from seropositive donors produce interferon-gamma (IFN-γ) by different mechanisms; one (T. gondii) dependent upon and the other (CMV) largely independent of interleukin-2 (IL-2) and its receptor. To determine whether IFN-γ-generating mechanisms unrelated to IL-2 also differ, we examined the requirement for accessory cells and their expressed or secreted products. In response to both specific antigens, IFN-γ secretion was strictly dependent upon the presence of accessory cells (monocytes), and was largely inhibited by monoclonal antibodies to class II (HLA-DR and -DQ) but not class I MHC antigens. Both CMV and T. gondii antigens stimulated monocytes to release interleukin-1 (IL-1), and IFN-γ production in response to both antigens was abolished by pretreatment with anti-IL-1 antibody. In contrast, the secretion of tumour necrosis factor (TNF) was not stimulated by either antigen, and IFN-γ production was not diminished by antisera directed at TNF-α or TNF-β. We conclude that CMV and T. gondii antigen-induced IFN-γ production requires a similar accessory cell mechanism; and that soluble antigen-stimulated IFN-γ secretion by human T4+ cells is dependent on monocytes, expression of class II MHC antigens, and the presence of IL-1. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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