| Abstrakt: |
Precursor feeding is a well-known strategy to enhance the commercial production of bioactive compounds in plant cell culture systems. For the first time, an effective in vitro platform was established for the enhanced production of phenylpropanoids with a precursor feeding approach in the cell suspension culture derived from friable leaf callus of Rhodiola imbricata. Precursor, phenylalanine feeding of 0.5 mM, 1 mM, 2 mM, and 3 mM concentrations were added to the cell suspension of R. imbricata and further, it was evaluated for the cell growth and production of phenylpropanoids (rosavin, rosarin, and p-coumaric acid). The suspension culture showed maximum cell growth (FW; 117 g/L, DW; 5.73 g/L) on day 15 of the incubation period in precursor untreated cells. Furthermore, cell suspension cultures treated with 1 mM phenylalanine accumulated the maximum biomass (FW; 136 g/L, DW; 6.70 g/L) on day 15. The enhanced rosavin (1.57 mg/g DW) and rosarin (0.78 mg/g DW) content were achieved on day 12 in 1 mM precursor concentration. Similarly, p-coumaric acid was detected maximum (2.50 mg/g DW) on day 12 in 3 mM precursor-treated suspension cultures of R. imbricata. The total phenolic content was improved significantly (43.72 mg/g DW) on day 12 in 2 mM phenylalanine treatment. Whereas, total flavonoid content was observed maximum (24.50 mg/g DW) on day 12 with 2 mM precursor concentration. The present work concluded that the precursor-mediated approach enhanced the overall phenylpropanoids production in the cell suspension culture of R. imbricata. Key message: The present investigation demonstrates the effect of phenylalanine precursor feeding; 0.5 mM, 1 mM, 2 mM, and 3 mM on cell growth, physiological characteristics, antioxidant activity, and metabolite yield for the first time in cell suspension culture derived from friable leaf callus of Rhodiola imbricata. [ABSTRACT FROM AUTHOR] |
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