Fluorescence identification of arthropathic calcium pyrophosphate single crystals using alizarin red S and a xanthene dipicolylamine ZnII complex.

Autor: Srinarawat, Waralee, Hemstapat, Ruedee, Tawonsawatruk, Tulyapruek, Patikarnmonthon, Nisa, Hamachi, Itaru, Ojida, Akio, Wongkongkatep, Jirarut
Předmět:
Zdroj: Analyst; 11/7/2022, Vol. 147 Issue 21, p4910-4918, 9p
Abstrakt: Calcium pyrophosphate deposition disease, previously known as pseudogout, is a type of chronic and painful joint arthropathy. Accurate identification of calcium pyrophosphate dihydrate (CPPD) single crystals is crucial for determining the best course of treatment. In this study, a two-step method involving alizarin red S (ARS) and a xanthene dipicolylamine ZnII (XDZ) complex was employed for the identification of CPPD single crystals in both triclinic and monoclinic forms using a fluorescence microscope and a microplate reader. The accurate identification method proposed in this study has the potential to advance the diagnosis and treatment of patients suffering from painful gouty arthritis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index