| Autor: |
Li, Pengfei, Peng, Furong, Zhang, Ruixuan, Yang, Yuheng, Yu, Yang, Bi, Chaowei |
| Předmět: |
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| Zdroj: |
Journal of Plant Diseases & Protection; Dec2022, Vol. 129 Issue 6, p1297-1304, 8p |
| Abstrakt: |
The plant pathogenic fungus Sclerotinia sclerotiorum infects more than 400 plant species worldwide. Boscalid, an inhibitor of succinate dehydrogenase in the electron transport chain of fungi, is highly effective in controlling S. sclerotiorum. S. sclerotiorum occurs widespread in Southwest China, where oilseed rape is grown. To assess the current status of boscalid resistance, the baseline sensitivity of boscalid was established on the basis of the effective concentration for 50% inhibition of mycelial growth (EC50) of 112 isolates of S. sclerotiorum collected from Sichuan in 2014. Results showed that the frequency distribution of EC50 of the 112 isolates was an approximate single-peak curve. The range of EC50 was 0.0090–0.2335 µg mL−1, and the mean EC50 was 0.0787 µg mL−1. On the basis of the discriminatory concentration in potato dextrose agar amended with 20 µg mL−1 of boscalid, a total of 399 single-sclerotium isolates of S. sclerotiorum were collected to monitor resistance from 14 counties. Results showed that the vast majority of strains are sensitive, accounting for 99.75%. Only one isolate with reduced boscalid sensitivity (BR strain ZX22) was found, which had a resistance factor of 34. Compared with sensitive isolates, ZX22 had faster radial growth, more ability to produce sclerotia, and higher virulence. DNA sequence analysis showed that ZX22 had the point mutant B-V58A (GTC to GCC) in the iron–sulfur protein subunit (SDHB), which might be the mutant sites that confer boscalid resistance to S. sclerotiorum. These results will deepen our understanding of boscalid's toxic effects and will be vital in designing resistance management strategies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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