Bioinformatics Analysis of MicroRNA Profiles Unveils Novel Biological Markers of Alzheimer's Disease.

Autor: Rahimpour, Alireza, Heidarzadehpilehrood, Roozbeh, Aghel, Majid, Jamalpoor, Zahra, Heydarian, Parichehr, Ghasemi, Seyed Abbas, Pirhoushiaran, Maryam
Zdroj: Neurochemical Journal; Sep2022, Vol. 16 Issue 3, p334-342, 9p
Abstrakt: Alzheimer's disease (AD) is a degenerative neurological disease that mostly affects the elderly in numerous countries all over the world. The miRNA expression profile of GSE157239, as well as the gene expression profiles of GSE33000, GSE122063, and GSE118553, were used to identify differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs) between AD patients and healthy controls, with the objective of determining miRNAs involved in the pathogenesis of Alzheimer's disease and recommending novel miRNA biomarkers in Alzheimer's patients. The detected DEMs' target genes were predicted using the mirTarBase and Tarbase databases and compared to the identified DEGs. Following that, 199 genes that overlapped between anticipated target genes from databases (mirTarBase and Tarbase) and discovered DEGs were approved as predicted target genes for DEMs. Funrich was also used to perform a Gene Ontology (GO) and pathway analysis for predicted target genes. Furthermore, the predicted target genes' Protein–Protein Interaction (PPI) was displayed using the Cytoscape platform. PPI analysis revealed ten hub-genes of the target genes. As a consequence, 199 DEGs and 22 DEMs were revealed to be differently expressed between AD patients and healthy controls. Remarkably, type 2 diabetes was the pathway with the highest concentration of target genes. The link between diabetes and Alzheimer's disease is thoroughly examined in the discussion section. Finally, the top detected DEMs among miRNAs were hsa-miR-125a-3p, hsa-miR-6131, hsa-miR-24-3p, hsa-miR-208a-5p, hsa-miR-6761-3p, and hsa-miR-3646. These findings significantly add to our understanding of the molecular pathways and associated miRNAs involved in the development of Alzheimer's disease. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: