| Autor: |
Teixeira Júnior, Antonio Augusto Lima, da Costa Melo, Syomara Pereira, Pinho, Jaqueline Diniz, Sobrinho, Thaís Bastos Moraes, Rocha, Thalita Moura Silva, Duarte, Denner Rodrigo Diniz, de Oliveira Barbosa, Liseana, Duarte, Wesliany Everton, de Castro Belfort, Marta Regina, Duarte, Kelly Gomes, da Silva Neto, Antonio Lima, de Ribamar Rodrigues Calixto, José, Paiva Paiva, Lúcio Cristiano, do Nascimento, Francisco Sérgio Moura Silva, Alencar Junior, Antonio Machado, Khayat, André Salim, da Graça Carvalhal Frazão Corrêa, Rita, Lages, Joyce Santos, dos Reis, Rodolfo Borges, Araújo Jr, Wilson Silva |
| Zdroj: |
BMC Cancer; 10/15/2022, Vol. 22 Issue 1, p1-22, 22p |
| Abstrakt: |
Background: Although penile cancer (PC) is uncommon in developed countries, it is widespread in developing countries. The state of Maranhão (Northeast, Brazil) has the highest global incidence recorded for PC, and, despite its socioeconomic vulnerability, it has been attributed to human papillomavirus (HPV) infection. This study aimed to determine the histopathological features, the prevalence of HPV infection, and the immunohistochemical profile of PC in Maranhão.Methods: A retrospective cohort of 200 PC cases were evaluated. HPV detection was performed using nested-PCR followed by direct sequencing for genotyping. Immunohistochemistry (IHC) was performed using monoclonal antibodies anti-p16INK4a, p53, and ki-67.Results: Our data revealed a delay of 17 months in diagnosis, a high rate of penile amputation (96.5%), and HPV infection (80.5%) in patients from Maranhão (Molecular detection). We demonstrated the high rate of HPV in PC also by histopathological and IHC analysis. Most patients presented koilocytosis (75.5%), which was associated with those reporting more than 10 different sexual partners during their lifetime (p = 0.001). IHC revealed frequent p16INK4a overexpression (26.0%) associated with basaloid (p < 0.001) and high-grade tumors (p = 0.008). Interestingly, p16 appears not to be a better prognostic factor in our disease-free survival analysis, as previously reported. We also demonstrated high ki-67 and p53 expression in a subset of cases, which was related to worse prognostic factors such as high-grade tumors, angiolymphatic and perineural invasion, and lymph node metastasis. We found a significant impact of high ki-67 (p = 0.002, log-rank) and p53 (p = 0.032, log-rank) expression on decreasing patients' survival, as well as grade, pT, stage, pattern, and depth of invasion (p < 0.05, log-rank).Conclusions: Our data reaffirmed the high incidence of HPV infection in PC cases from Maranhão and offer new insights into potential factors that may contribute to the high PC incidence in the region. We highlighted the possible association of HPV with worse clinical prognosis factors, differently from what was observed in other regions. Furthermore, our IHC analysis reinforces p16, ki-67, and p53 expression as important diagnosis and/or prognosis biomarkers, potentially used in the clinical setting in emerging countries such as Brazil. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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