| Autor: |
Xiuqin Feng, Rahman, Md. Mostafijur, Qian Hu, Bang Wang, Hassan Karim, Guzmán, Carlos, Harwood, Wendy, Qiang Xu, Yazhou Zhang, Huaping Tang, Yunfeng Jiang, Pengfei Qi, Mei Deng, Jian Ma, Jingyu Lan, Jirui Wang, Guoyue Chen, Xiujin Lan, Yuming Wei, Youliang Zheng |
| Předmět: |
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| Zdroj: |
Frontiers in Plant Science; 9/23/2022, Vol. 13, p1-13, 13p |
| Abstrakt: |
Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx-1 in the mutant (M2-1105). The G1086A mutation is located at the 3' splicing receptor (AG) site of the fourth intron, resulting in an abnormal RNA splicing. The A2424G mutation was a synonymous mutation in the ninth intron. The pre-mRNA of Wx-1 was incorrectly spliced and transcribed into two abnormal transcripts. The type I transcript had a 6 bp deletion in the 5' of fifth exon, leading to a translated HvGBSSI protein lacking two amino acids with a decreased starch-binding capacity. In the type II transcript, the fourth intron was incorrectly cleaved and retained, resulting in the premature termination of the barley Wx-1 gene. The mutations in the Wx-1 decreased the enzymatic activity of the HvGBSSI enzyme and resulted in a decreased level in amylose content. This work sheds light on a new Wx-1 gene inaction mechanism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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