| Autor: |
Bairamukov, V. Yu., Filatov, M. V., Kovalev, R. A., Pantina, R. A., Grigoriev, S. V., Varfolomeeva, E. Yu. |
| Zdroj: |
Journal of Surface Investigation: X-Ray, Synchrotron & Neutron Techniques; Oct2022, Vol. 16 Issue 5, p854-859, 6p |
| Abstrakt: |
The HeLa cell's nuclei surface landscape, which appeared after the mechanical deformation, was studied by atomic-force microscopy. Nuclei were isolated from HeLa-line cells, and deformed under centrifugal forces, followed by fixation with glutaraldehyde. The resulting landscape was shown to be mainly due to the high chromatin resistance to deformation. This stability correlates with DNA supercoiling. Cells' treatment with topoisomerase inhibitors I and II leads to the relaxation of supercoiling and significant flattening of the nuclei. On the contrary, cells' treatment with a DNA intercalator leads to an increase in the DNA rigidity and, as a result, the resistance of chromatin to mechanical deformation. Thus, the observed changes in morphology reflect the functional features of the cell nucleus. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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