| Abstrakt: |
Ferulago (Ferulago ssp.) genus as medicinal plant belongs to Apiaceae family. It is endemic in Iran. Excess harvesting and environmental stresses such as drought, lead to as endangered plant. By considering this plant as a valuable medicinal-rangeland plant, its production and reclamation is an essential task. This investigation was conducted to optimize micropropagation of two endemic species of Ferulago, included F. angulate and F. subvelutina. Higher percentage of callus induction in F. angulata specie was obtained as 100%, using rootlet explant (under treatments of 0 BAP +1 NAA, 0 BAP +1.5 NAA and 0 BAP +2 NAA mg/l) and also true leaf explants (under treatments of 0.5 BAP + 0.5 NAA and 1 BAP +2 NAA mg/l). For F. subvelutina, higher callus induction was obtained using true leaf explants (under treatments of 0.5 BAP + 0.5 NAA and 1 BAP + 2 NAA mg/l), as 72.33 and 71.66%, respectively. The combined application of the cytokinin and auxin phytohormones was effective in regenerating of both species; so that, the higher regeneration was obtained as 53.33 and 46.66% in F. angulate under treatments of 0.5 BAP + 0.2 NAA mg/l and 0.5 BAP + 0.4 NAA mg/l, respectively. Similarly, the higher regeneration as 53.33 and 60% was obtained in F. subvelutina under treatments of 1 BAP+0.2 NAA mg/l and 1 BAP+0.4 NAA mg/l, respectively. The best rooting treatment in both species was determined as the ½ MS cultural medium included 0.5 mg/l IBA. Their adaptation stage were done using mixture of soil, peat soil and sand with a weight ratio of 1: 1: 1, in which the total of 80% of regenerated plants from tissue culture, were successfully adapted to the outside environment. [ABSTRACT FROM AUTHOR] |
