| Abstrakt: |
[Objectives] To establish the quality standard for Euonymi Grandiflorus Caulis and Folium. [Methods] Qualitative identification was performed by methods of origin identification, microscopic identification and thin layer chromatography (TLC); the moisture, total ash, acid-insoluble ash, and extract were detected using the method in the Chinese Pharmacopoeia (2020 Edition), and the contents of quercetin and kaempferol were determined by High Performance Liquid Chromatography (HPLC). [Results] The leaves of Euonymi Grandiflorus were subleathery, narrow and long elliptic or narrow obovate with cyme, and capsules were often with narrow wing ridge. Characteristics of microscopic identification were significant. Calcium oxalate cluster crystals, cubic crystals, inlaid parenchyma cells, starch granules, stomata, and fiber bundles could be observed. A TLC method for identification of Euonymi Grandiflorus Caulis and Folium was established. The contents of water, total ash, acid - insoluble ash were not higher than 13%, 9%, 12%, respectively; the extract were no less were not higher than 13%, 9%, 12% and 17% in ten batches of samples, respectively; the sum of quercetin and kaempferol were no less than 0.20%. [Conclusions] The quality standard of Euonymi Grandiflorus Caulis and Folium were established through the research. This method is accurate, specific, and reproducible and can be used for the quality control of Euonymi Grandiflorus Caulis and Folium. [ABSTRACT FROM AUTHOR] |
