STUDIES IN VITRO ON THE REACTION OF PERITONEAL EXUDATE CELLS FROM MICE IMMUNE TO INFECTION WITH NEMATOSPIROIDES DUBIUS WITH THE INFECTIVE THIRD STAGE LARVAE OF THIS PARASITE.

Autor: Chaicumpa, V., Jenkin, C. R.
Zdroj: Australian Journal of Experimental Biology & Medical Science; Feb1978, Vol. 56 Issue 1, p61-68, 8p
Abstrakt: This article informs that using millipore chambers, peritoneal exudate cells of mice immune to infection with Nematospiroides dubius have a marked effect on the infectivity of the third stage larvae. In a study, Peritoneal exudate cells from immune mice were collected 7 days after the last intravenous injection of living larvae and monolayers prepared in Leighton tubes. Leighton tubes containing 0.6 ml of 199 medium were divided into three groups of six. To each tube in Group 1 was added 0.l ml of specific antiserum, to Group 2, 0.1 ml of serum from normal mice, whilst to Group 3, 0.1 ml of 199 medium. The suspended larvae from each tube were then fed to the respective mice. All animals were killed 5 days after challenge and the number of cysts counted.
Databáze: Complementary Index