| Autor: |
Napel, Chr. H. H. Ten, The, T. H., Bijker, J., de Gast, G. C., Langenhuysen, M. M. A. C. |
| Předmět: |
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| Zdroj: |
Clinical & Experimental Immunology; Jul1977, Vol. 29 Issue 1, p52-60, 9p |
| Abstrakt: |
A cytomegalovirus (CMV) induced lymphocyte transformation test (LTT) performed as a microtechnique assay is described. Investigations were done in a group of twenty-five healthy adults with normal lymphocyte reactivity to phytohaemagglutinin and previously encountered antigens. Lymphocyte reactivity was established in CMV-seropositive persons using heat-inactivated cell-free CMV strain AD 169 in an optimal stimulating dose of 101.5 TCID50. Positive CMV-LTT results were shown to be induced by purified inactivated CMV. Most persons (twelve out of seventeen) with antibodies against CMV-induced late or virus-structure antigens (LA) showed positive (stimulation index > 2.0) CMV-LTT results. Magnitude of CMV-LTT responses was positively related to the CMV-LA Ab titres (P<0.01). All persons (six) with antibodies against CMV early or non-structural antigens showed positive CMV-LTT results and highest stimulation rates were found in this group. Generally CMV- seronegative persons were non-responsive. Only in one case (LA Ab titre 10) a borderline positive response (stimulation index 2.3) was seen. The CMV-LTT was shown to have virus-specific properties because it was not related to simultaneously performed LTTs with Epstein-Barr virus and Herpes simplex virus in the same persons. It is concluded that CMV-LTTs are useful virus-specific in vitro techniques for the study of CMV-directed lymphocyte reactivity. Its possible relationship to other cell-mediated immunity tests and its practical applications are discussed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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