| Autor: |
Laminet, A. A., Kumamoto, C. A., Plückthun, A. |
| Předmět: |
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| Zdroj: |
Molecular Microbiology; Jan1991, Vol. 5 Issue 1, p117-122, 6p, 1 Diagram, 2 Charts, 1 Graph |
| Abstrakt: |
The rate of folding of the precursor of β-lactamase is not influenced by the presence of SecB under conditions in which GroEL/ES retards the folding. Wild-type (β-lactamase and several mutants in the signal or the mature protein, affecting either transport or enzyme kinetics and probably folding, were examined for total expression, total enzymatic activity, and transported β-lactamase (in vivo resistance) in secB and secB+ strains. We conclude that there is no indication of any relevant interaction between SecB- and pre-p-lactamase in vitro, nor did the secB+ mutation affect the transport of wild-type β-lactamase or any of the mutants in vivo. Thus, putative Escherichia coli 'folding modulators' must be of limited specificity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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