DNA Barcoding on Indian Ocean Squid, Uroteuthis duvaucelii (D'Orbigny, 1835) (Cephalopoda: Loliginidae) from the Java Sea, Indonesia.

Autor: Afiati, Norma, Subagiyo, Handayani, Christina Retna, Hartati, Retno, Kholilah, Nenik
Předmět:
Zdroj: Scientific Journal of Fisheries & Marine / Jurnal Ilmiah Perikanan dan Kelautan; Nov2022, Vol. 14 Issue 2, p231-245, 15p
Abstrakt: Uroteuthis duvaucelii (D' Orbigny, 1835) also known as the Indian Ocean Squid is a highly important commercial marine organism along the Java coast, Indonesia. Based on genetic variation this species-complex are polymorphic and cryptic. In the present study, the genetic diversity and stock structure of loliginid squid U. duvaucelii is investigated using a mitochondrial gene cytochrome oxidase subunit I (COI). Samples were collected by hand-jigging onboard of an 8hp small fisher-boat equipped with a few lamps during May to August 2015, May 2016 and August to November 2018. Sample collection started at dusk until midnight. The attractor was a weighed-quill attached to nylon string, manually immersed into the water and pulled quickly and continuously for about 3-5 minutes at each effort. The determination was conducted with BLAST. Phylogenetic analysis showed two separate clusters with 100% bootstrap value, in which cluster II from Palabuhanratu has divergences of 5.9 - 7.0%, compared to cluster I. Genetic variations exist within and among individuals over the locations. Palabuhanratu individuals have the highest polymorphism levels compared to other locations as shown by 40 segregating sites (S). Frequencies of A, C, G, and T in mtDNA of the 20 specimens U. duvaucelii are biased toward A and T, with T being the most favoured and G being the least favoured nucleotide. All specimen showed no amino acid frequency for glutamic acid and all four individuals in cluster II (south coast) also have no amino acid frequencies for aspartic acid and valine as well. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index