Anti-Biofilm, Antibacterial, and Anti-Quorum Sensing Activities of Selected South African Plants Traditionally Used to Treat Diarrhoea.
Autor: | Adeyemo, Rasheed Omotayo, Famuyide, Ibukun Michael, Dzoyem, Jean Paul, Lyndy Joy, McGaw |
---|---|
Předmět: |
THERAPEUTIC use of plant extracts
STATISTICS DIARRHEA MEDICINAL plants SOLVENTS AFRICAN traditional medicine ONE-way analysis of variance ANTI-infective agents BIOFILMS HEALTH outcome assessment DESCRIPTIVE statistics PLANT extracts CELL surface antigens DATA analysis software DATA analysis IMMUNODIAGNOSIS |
Zdroj: | Evidence-based Complementary & Alternative Medicine (eCAM); 9/28/2022, p1-12, 12p, 4 Charts, 2 Graphs |
Abstrakt: | The development of resistance of microorganisms to conventional antibiotics is a major global health concern; hence, there is an increasing interest in medicinal plants as a therapeutic option. This study aimed to evaluate the antibacterial, anti-biofilm, and anti-quorum activities of crude extracts prepared using various solvents of nine indigenous South African plants used locally for the treatment of diarrhoea. The minimum inhibitory concentration (MIC) was determined using the broth microdilution method and the crystal violet assay was used to test the anti-biofilm activity of the extracts against a panel of bacteria. Anti-quorum sensing activity of the extracts was assessed via inhibition of violacein production in Chromobacterium violaceum ATCC 12472. Preliminary screening of extracts against E. coli ATCC 25922 revealed that the acetone extracts had significant activity, with MIC values ranging from 0.04 to 0.63 mg/mL. Further screening against a panel of bacterial pathogens showed that the acetone extract of Bauhinia bowkeri was the most active with MIC of 0.01 mg/mL against Salmonella enteritidis, followed by Searsia lancea with MIC of 0.03 mg/mL against Bacillus cereus. All the plant extracts prevented the attachment of biofilms by more than 50% against at least one of the tested bacteria. However, only the mature biofilm of B. cereus was susceptible to the extracts, with 98.22% eradication by Searsia pendulina extract. The minimum quorum sensing inhibitory concentration of the extracts ranged from 0.08 to 0.32 mg/mL with S. lancea having the most significant activity. The extract of S. lancea had the best violacein production inhibitory activity with IC |
Databáze: | Complementary Index |
Externí odkaz: |