| Autor: |
Natalia, Kristina, Wahjudi, Mariana, Kok, Tjie |
| Předmět: |
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| Zdroj: |
Journal of Aquaculture & Fish Health; Sep2022, Vol. 11 Issue 3, p391-401, 11p |
| Abstrakt: |
Fishery products are types of commodity with various levels of development including the addition of food additives. They are very susceptible to pathogenic microbial contamination hence they can cause food poisoning and disease outbreaks in consumers. In this study, tuna fish spiked with Salmonella spp., Escherichia coli, and Listeria monocytogenes bacteria is examined for bacteria contamination. The examination of these pathogenic bacteria is carried out using multiplex PCR (mPCR), a modern technique developed to detect target DNAs of bacteria simultaneously. The results of mPCR are compared to those of biochemical tests for sensitivity and time-efficiency. Based on the results obtained, the mPCR method is +1250-fold more sensitive and +80-fold more time efficient than the biochemical test. Therefore, mPCR method could be recommended to be used as a tool for the detection of bacteria in fishery products. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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