| Autor: |
Smoczynska, Aleksandra, Pacak, Andrzej, Grabowska, Aleksandra, Bielewicz, Dawid, Zadworny, Marcin, Singh, Kashmir, Dolata, Jakub, Bajczyk, Mateusz, Nuc, Przemyslaw, Kesy, Jacek, Wozniak, Magdalena, Ratajczak, Izabela, Harwood, Wendy, Karlowski, Wojciech M., Jarmolowski, Artur, Szweykowska-Kulinska, Zofia |
| Předmět: |
|
| Zdroj: |
Frontiers in Plant Science; 9/12/2022, Vol. 13, p1-19, 19p |
| Abstrakt: |
Nitrogen (N) is an important element for plant growth and development. Although several studies have examined plants' response to N deficiency, studies on plants' response to excess N, which is common in fertilizerbased agrosystems, are limited. Therefore, the aim of this study was to examine the response of barley to excess N conditions, specifically the root response. Additionally, genomic mechanism of excess N response in barley was elucidated using transcriptomic technologies. The results of the study showed that barley MADS27 transcription factor was mainly expressed in the roots and its gene contained N-responsive cis-regulatory elements in the promoter region. Additionally, there was a significant decrease in HvMADS27 expression under excess N condition; however, its expression was not significantly affected under low N condition. Phenotypic analysis of the root system of HvMADS27 knockdown and overexpressing barley plants revealed that HvMADS27 regulates barley root architecture under excess N stress. Further analysis of wild-type (WT) and transgenic barley plants (hvmads27 kd and hvmads27 c-Myc OE) revealed that HvMADS27 regulates the expression of HvBG1 ß-glucosidase, which in turn regulates abscisic acid (ABA) level in roots. Overall, the findings of this study showed that HvMADS27 expression is downregulated in barley roots under excess N stress, which induces HvBG1 expression, leading to the release of ABA from ABA-glucose conjugate, and consequent shortening of the roots. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
